Journal of Global Infectious Diseases

ORIGINAL ARTICLE
Year
: 2018  |  Volume : 10  |  Issue : 2  |  Page : 67--73

Clonal lineage diversity, antibiotic resistance, and virulence determinants among methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolated from nurses at a teaching hospital in Ilam, Iran: Successful nares decolonization by mupirocin


Ali Hematian1, Aazam Monjezi1, Ramin Abiri2, Parviz Mohajeri2, Abbas Farahani3, Setareh Soroush4, Morovat Taherikalani5 
1 Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran
2 Department of Microbiology, Kermanshah University of Medical Sciences, Kermanshah, Iran
3 Department of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
4 Department of Microbiology, School of Medicine and Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Lorestan, Iran
5 Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam; Department of Microbiology, School of Medicine and Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Lorestan, Iran

Correspondence Address:
Prof. Morovat Taherikalani
Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Department of Microbiology, School of Medicine and Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad
Iran

Background: Staphylococcus aureus is known to be responsible for nosocomial infections, and the typing method was useful in managing the reservoir of bacteria. The main aim of this study was to determine the prevalence of S. aureus in the nares and hands of nurses working in Imam Khomeini hospital, Ilam, Iran, as well as to determine the clonal relatedness, antimicrobial susceptibility profiles, different virulence, and resistance determinants among these isolates. The evolution of mupirocin activity in the eradication of methicillin-resistant S. aureus (MRSA) and methicillin-susceptible S. aureus (MSSA) colonization in the nares of the healthcare workers in Ilam, Iran, was also determined in this study.Materials and Methods: In this cross-sectional study, 80 nurses, auxiliary nurses, and service workers from Imam Khomeini Hospital were enrolled. MRSA, antibiotic susceptibility, and virulence determinants were evaluated. Then, the isolates were subjected to pulsed field gel electrophoresis (PFGE) and Staphylococcal cassette chromosome mec typing. Results: Our results demonstrated that 23% of isolates were MRSA. PFGE results demonstrated that pulsotypes A (3 out of 30; 10%) and J (3 out of 30; 10%), pulsotypes E (2 out of 30; 6.7%), M (2 out of 30; 6.7%), P(2 out of 30; 6.7%), and V (2 out of 30; 6.7%) were the most predominant pulsotypes, respectively. Conclusion: We cannot give conclusive suggestions about the correlation between nasal carriage and infections, but we suggest the monitoring of all healthcare workers annually, decontamination of their noses by using mupirocin and other antistaphylococcal agents, and also the washing of their hands at least every 2 h.


How to cite this article:
Hematian A, Monjezi A, Abiri R, Mohajeri P, Farahani A, Soroush S, Taherikalani M. Clonal lineage diversity, antibiotic resistance, and virulence determinants among methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolated from nurses at a teaching hospital in Ilam, Iran: Successful nares decolonization by mupirocin.J Global Infect Dis 2018;10:67-73


How to cite this URL:
Hematian A, Monjezi A, Abiri R, Mohajeri P, Farahani A, Soroush S, Taherikalani M. Clonal lineage diversity, antibiotic resistance, and virulence determinants among methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolated from nurses at a teaching hospital in Ilam, Iran: Successful nares decolonization by mupirocin. J Global Infect Dis [serial online] 2018 [cited 2020 Dec 4 ];10:67-73
Available from: https://www.jgid.org/article.asp?issn=0974-777X;year=2018;volume=10;issue=2;spage=67;epage=73;aulast=Hematian;type=0