Journal of Global Infectious Diseases

ORIGINAL ARTICLE
Year
: 2014  |  Volume : 6  |  Issue : 3  |  Page : 109--113

Clinical applicability of various dengue diagnostic tests in resource-limited endemic settings


Baijayantimala Mishra, Puneet K Gupta, Vandana Dhiman, Sujit K Pujhari, Mirnalini Sharma, Radha K Ratho 
 Department of Virology, Post Graduate Institute of Medical Education and Research, Chandigarh, India

Correspondence Address:
Baijayantimala Mishra
Department of Virology, Post Graduate Institute of Medical Education and Research, Chandigarh
India

Introduction: Dengue is one of the most important arboviral infections caused by one of the four dengue serotypes, 1-4. Objective: To study the applicability of different diagnostic methods in diagnosis of dengue viral infection. Materials and Methods: A total of 2101 blood samples were collected for confirmation of dengue viral infection. All the samples were tested by dengue-specific IgM ELISA, of which 111 were also tested for NS1 antigen detection and 27 acute samples (≤5 days) were further subjected for viral RNA detection by RT-PCR and isolation in C6/36 cell line. To detect the sensitivity of NS1 antigen for different dengue virus serotypes, four dengue serotype 1 and 12 dengue 3 were subjected for the NS1 antigen assay. Results: Most common age group affected was 16-45 years, with male to female ratio of 2.8:1. During first 3 days of illness virus isolation and RT-PCR were the most sensitive (83%) followed by NS1 antigen detection (75%) and IgM detection (37.5%). The positivity of IgM detection was found to be significantly higher as compared to NS1 detection during 4 to 5 days and also after 5 days of illness (P < 0.05). Dengue serotypes 1 and 3 were found to be co-circulated, dengue 1 being the predominant serotype. Conclusion: Virus isolation and RT-PCR were the most sensitive tests during the early period of illness whereas beyond third day, IgM antibody detection was found to be the most sensitive method of dengue diagnosis.


How to cite this article:
Mishra B, Gupta PK, Dhiman V, Pujhari SK, Sharma M, Ratho RK. Clinical applicability of various dengue diagnostic tests in resource-limited endemic settings .J Global Infect Dis 2014;6:109-113


How to cite this URL:
Mishra B, Gupta PK, Dhiman V, Pujhari SK, Sharma M, Ratho RK. Clinical applicability of various dengue diagnostic tests in resource-limited endemic settings . J Global Infect Dis [serial online] 2014 [cited 2021 Jan 18 ];6:109-113
Available from: https://www.jgid.org/article.asp?issn=0974-777X;year=2014;volume=6;issue=3;spage=109;epage=113;aulast=Mishra;type=0