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ORIGINAL ARTICLE
Year : 2018  |  Volume : 10  |  Issue : 2  |  Page : 84-88

Isolation and characterization of novel broad host range bacteriophages of Vibrio cholerae O1 from Bengal

Sounak Sarkar1, Mayukh Das2, Tushar Suvra Bhowmick3, Hemanta Koley1, Robert Atterbury3, Alok K Chakrabarti1, Banwarilal L Sarkar1
1 Division of Bacteriology, Vibrio Phage Reference Laboratory, National Institute of Cholera and Enteric Diseases, Kolkata, West Bengal, India
2 Department of Plant Pathology and Microbiology, Texas Aandm University, Texas, USA
3 Department of Veterinary infectioua Diseases, School of Veterinary Medicine and Science, The University of Nottingham, Leicestershire, UK

Correspondence Address:
Dr. Banwarilal L Sarkar
Division of Bacteriology, Vibrio Phage Reference Laboratory, National Institute of Cholera and Enteric Diseases (ICMR), P-33, CIT Road, Scheme XM, Kolkata - 700 010, West Bengal
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/jgid.jgid_37_17

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Objectives: We have isolated a total of five newer cholera phages which are novel broad host range to incorporate with the existing phage typing schemes for an extended typing scheme. Materials and Methods: These newly isolated phages were well characterized including the electron micrograph. A total of 300 Vibrio cholerae strains were isolated from the different endemic region in India were included in phage typing study. Results: These phages were found different from the existing phages. Electron microscopic results showed that the phages belonged to myophage and podophage group. Characterization of the phages based on pH, temperature, and organic solvent sensitivity showed differences among the phages used in this study. All the strains of Vibrio O1 were typeable (100%) with the five set of cholera phages. Of these, 40% strains were clustered under Type-1. Conclusion: The newer Vibrio phages are novel and broad host range and will be useful to incorporate with the existing phage typing system for more precisely discriminate the strains of Vibrio cholerae.


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