| Abstract|| |
Background: Pediculosis capitis is a ubiquitous parasitic skin disease associated with intense pruritus of the scalp. In developing countries it frequently affects children and adults, but epidemiological data at the community level are rare. Objectives: To assess prevalence and risk factors associated with pediculosis capitis in a resource-poor community in Lima, Peru. Materials and Methods: In total, 736 persons living in 199 households in a circumscribed neighbourhood were examined for head lice and nits by visual inspection. At the same time, socio-demographic data were collected using a structured questionnaire. Variables associated with pediculosis were identified by performing a bivariate analysis, followed by a multivariate logistic regression analysis. Results: Prevalence of pediculosis capitis was 9.1% (95% confidence interval (CI): 7.0-11.2 %) in the general population and 19.9% (CI: 15.4-24.4%) in children ≤15 years of age. Multivariate analysis showed that pediculosis capitis was significantly associated with age ≤ 15 years (OR: 16.85; CI:7.42-38.24), female sex (OR: 2.84; CI: 1.58-5.12), household size of >4 persons (OR: 1.98; CI: 1.11-3.55), low quality of house construction material (OR:2.22; CI: 1.20-4.12), and presence of animals in the household (OR: 1.94; CI: 1.11-3.39). Conclusion: Pediculosis capitis was a very common disease in the studied community in Lima, Peru. Our logistic regression analysis affirms that young age is the most important risk factor for pediculosis capitis. Moreover, female sex, large household size, living in wooden houses and the presence of animals were identified as being significantly associated with head lice infestation.
Keywords: Ectoparasites, Epidemiology, Head lice, Logistic regression analysis, Pediculosis capitis, Peru, Poverty, Risk factors
|How to cite this article:|
Lesshafft H, Baier A, Guerra H, Terashima A, Feldmeier H. Prevalence and risk factors associated with pediculosis capitis in an impoverished urban community in Lima, Peru. J Global Infect Dis 2013;5:138-43
|How to cite this URL:|
Lesshafft H, Baier A, Guerra H, Terashima A, Feldmeier H. Prevalence and risk factors associated with pediculosis capitis in an impoverished urban community in Lima, Peru. J Global Infect Dis [serial online] 2013 [cited 2020 Nov 24];5:138-43. Available from: https://www.jgid.org/text.asp?2013/5/4/138/121994
| Introduction|| |
Pediculosis capitis is an epidermal parasitic disease caused by infestation of the scalp with head lice (Pediculus humanus capitis, De Geer 1778). It is a ubiquitous disease in children  and is especially frequent in resource-poor communities in developing countries, where prevalences of around 40% have been reported in some studies. ,,,, The main symptom of pediculosis capitis is pruritus of the scalp, but excoriations, impetiginization and cervical lymphadenopathy may also be present. , Intense itching with subsequent sleep disturbances may result in concentration difficulties and poor performance in school.  Transmission occurs by intimate head-to-head contact and theoretically also through sharing fomites (hair brushes, combs, towels and bedding); however, the importance of the latter route of transmission remains a matter of debate. ,
Together with scabies, cutaneous larva migrans and tungiasis, pediculosis capitis belongs to the family of neglected poverty-associated skin parasitoses. 
Hitherto, there are few studies on the occurrence and risk factors of pediculosis capitis in resource-poor settings in the developing world. In Peru, a few epidemiological studies have been performed in 1990s, ,, but recent data do not exist. The high prevalence observed in these studies is a matter of concern, especially since head lice are suspected of transmitting rickettsia, and endemic typhus occurs in some areas of Peru. 
Our community-based study aims at describing the prevalence and risk factors of pediculosis capitis in an impoverished neighborhood in the center of Lima, Peru.
| Materials and Methods|| |
The study was performed in the urban neighborhood Barrios Altos, a typical resource-poor community in Lima, Peru. The community is situated in Lima's historical center and has a population of about 290,000 persons. Population density is high (Instituto Nacional de Estadνstica e Informαtica census 2005). In Barrios Altos, most people live in decayed so-called quintas, complexes of old adobe houses with backyards and small alleys. Adjacent to the quintas some people have built small wooden dwellings. Sanitation is insufficient and living conditions are precarious.
Out of the various impoverished communities in Lima, Barrios Altos was chosen because it was easily accessible and considerably safe for the investigators. Furthermore, at the time of the study the Universidad Peruana Cayetano Heredia (UPCH) used to run a center for children and health care, called Casa Carrillo Maϊrtua, in the neighborhood, which served as a contact base with the population. Five quintas in the area were considered to be relatively safe by our local assistants and were therefore chosen for the study. They were inhabited by 840 individuals belonging to 199 households.
Contact with the local population and neighborhood associations was made through the Casa Carrillo Maϊrtua in Barrios Altos. From January to March 2006, during the summer season, all 199 households of the five quintas were visited in a door-to-door-survey. To increase the acceptability of the survey, the clinical investigator (A.B.) was always accompanied by a female volunteer from the neighborhood. When a family member was absent, the household was visited a second time. If still absent, the person was invited to be examined the following weekend in the parish hall.
The entire scalp and hair shafts of each person were carefully inspected for the presence of pediculosis capitis. Active or recently experienced pediculosis was diagnosed when nymphs, adult lice, or nits were visible. Socio-demographic and clinical information was assessed using two pre-tested questionnaires: one for the household (number of persons living together, number of beds in the household and presence of animals) and one for the individual (sex, age, marital status and presence of head lice).
Permission for the study was obtained from the Institutional Ethics Committee of the UPCH in Lima. All participants or, in the case of minors, their legal guardians provided informed written consent.
When pediculosis capitis was diagnosed, patients or care-takers received a shampoo containing permethrin 1% (Nopucid, Farmindustria, Lima, Peru) and a lice-comb. They were instructed to treat all household members as follows: Apply the shampoo to wet hair for 10 minutes, then comb the hair thoroughly with the lice-comb and rinse. It was recommended to repeat the procedure after 3-6 days and patients as well as care-persons were informed about the nature of the disease and its transmission.
For data storage and statistical analysis, we used Statistical Package for the Social Sciences (SPSS) for Windows (version 15.0 SPSS Inc., Illinois, USA). Data were entered twice into the database and were checked for entry-related errors. We applied Fisher΄s exact test to determine the significance of differences in relative frequencies and assessed odds ratios (ORs) with 95% confidence intervals (CIs). Variables that were significant (P < 0.05) in bivariate analysis entered a logistic regression to identify independent associated factors. In order to avoid multicollinearity, the variable 'beds per person' was excluded from logistic regression because of its connection with the variable 'number of persons in the household'. The Omnibus test was applied to test model performance. The adjusted ORs of the multivariate regression analysis were calculated with 95% CI.
| Results|| |
In the door-to-door survey, we collected clinical and socio-demographic data of 736 out of 840 persons (87.6%) from all 199 households in the study area. The proportion of children ≤15 years was 41.0% (n = 302) [Table 1]. The median number of persons per household was 5 (range: 1-14; interquartile range (IQR): 4-6). About two-thirds (65.5%) of the households had 3 beds (range: 1-9; IQR: 2-3). The median number of beds per person was 0.56 (range: 0.2-1; IQR: 0.4-0.75). The houses were made from adobe (77.4%; n = 154) or wood (22.6%; n = 45) and usually had a concrete floor (94.8%). More than half of the households (58.3%; n = 116) used a communal water source while the others had private water supply.
Pediculosis capitis was present in 67 of the 736 participants (9.1%; CI: 7.0-11.2%). Among children ≤15 years, 60 out of 302 (19.9%; CI: 15.4-24.4%) were affected. Forty-three out of 202 (21.3%; CI: 15.7-26.9%) school children between 5 and 15 years presented signs of pediculosis capitis. Peak prevalence was found in the age group of 8-11 years with 23 cases among 78 children (29.5%; CI: 20.1-44.3%). [Table 1] and [Figure 1] show the results according to different age groups.
|Table 1: Prevalence of pediculosis capitis according to age (in years). N=736|
Click here to view
The bivariate analysis showed that head lice infestation was significantly associated with female sex, young age, household size >4 persons, a low ratio of beds per person, wooden houses and presence of animals (dogs and cats) in the household [Table 2].
|Table 2: Bivariate analysis of exposure variables associated with pediculosis capitis (n=736)|
Click here to view
In the logistic regression analysis, we identified age ≤15 years, female sex, household size >4 persons, wooden houses and the presence of animals as factors associated with pediculosis capitis [Table 3]. Young age was shown to be by far the most important risk factor, with an adjusted OR of 16.9 between children ≤15 years and older individuals [Table 3].
|Table 3: Logistic regression analysis for risk factors for pediculosis capitis|
Click here to view
The Omnibus test (Chi-square 108.8; P <0.001) showed good overall fit of the logistic regression.
| Discussion|| |
The overall prevalence of pediculosis capitis found in our study area was 9.1% and therewith lower than prevalences observed in population-based studies in resource-poor settings in Brazil (43%)  and Egypt (19%),  but higher than in a study in a rural community in Tanzania (5.3%).  However, the age distribution in these studies was similar to our findings, with school-age children being the most affected group. ,, The high prevalence among school children is generally ascribed to facilitated transmission of head lice during head-to-head contact while children study and play in groups.  The pediculosis prevalence of 21.3% in school children in our study is lower than the frequencies observed in previous studies on school children in Peru (39.4-49.0%) ,, and other South American Countries, namely Venezuela (28.8%),  Argentina (29.7% and 61.4%) , and Brazil (35%).  Lower prevalences between 1.8% and 13.3% were observed in studies on school children in Turkey, Yemen, Iran and Korea. ,,,, The considerable variation may be partly related to a different sensitivity of the diagnostic methods used in these studies. Furthermore, the incidence of head lice infestation may vary according to the season of the year  and pediculosis capitis often occurs in epidemics.  Obviously, the point of time of a cross-sectional study of pediculosis capitis will influence the prevalence. Hence, prevalence of head lice infestation in different settings can only be compared if the studies are performed during, for instance, peak transmission season.
We found girls to be more frequently affected by pediculosis capitis than boys (female: Male ratio 2.2:1, adjusted OR 2.84, P < 0.001) [Table 3]. This corroborates previous findings where female:male ratios varied between 2:1 and 10:1. ,,,,,, The preponderance of girls reflects the observation that they tend to have closer and more frequent head-to-head contact among each other than boys.  Moreover, they usually have longer hair than boys, thereby increasing the odds that during head contact the head lice can grasp a hair and migrate from one host to another. However, whether hair length indeed affects pediculosis capitis infestation is still discussed controversially in the literature. ,,,
Our finding that living in a household with >4 persons is a significant risk factor for head lice is in accord with studies from Greece, Malaysia and Iran, which show a significant positive association of family size with the occurrence of pediculosis capitis. ,, In the bivariate analysis, the number of beds per person was found to significantly predict pediculosis capitis [Table 2]. Sharing beds is a sign of crowding and facilitates the direct and indirect head lice transmission through head-to-head contact and fomites. In concurrence, previous studies in Venezuela, Yemen and Iran also showed that sharing beds increased risk of pediculosis. ,,
It is difficult to explain why the presence of animals (dogs, cats) and houses constructed from wood were identified as independent associated factors. The insect's entire life cycle takes place on the human head and animal reservoirs do not exist.  The families who owned animals were not significantly larger than those who did not (mean = 3.85 persons vs. 3.75 persons respectively, P = 0.66).
Living in makeshift wooden houses indicates a lower socio-economic status this area, where most people live in older, run-down adobe houses. In developing countries, pediculosis capitis is considered a poverty-associated disease  and an association with the low economic status has been demonstrated in previous studies. ,,, This may explain the link between house construction material and head lice infestation.
The prevalence of head lice in our study population may be underestimated as visual inspection is a rather insensitive tool to diagnose active infestation; , even though, it is applied in most pediculosis capitis studies. On the other hand, we defined the presence of nits as a diagnostic criterion although nits in fact only prove recent, but not necessarily active infestation. 
In the study population >15 years of age, men were underrepresented as they were often at work during our visits. Since they are not considered a risk group for pediculosis capitis, the overall prevalence in the community may be slightly overestimated.
The fact that Barrios Altos was chosen as a study site that was considerably safe for the investigators in terms of criminality indicates that in other areas social and safety standards are even poorer. In these communities, head lice infestation may supposedly be even more common.
| Main Conclusions|| |
With an overall prevalence of 9.1%, pediculosis capitis was a very common disease in the studied community. Although studies from different countries show great variation in head lice prevalence, the age distribution seems to be very stable, with school children being the most affected age group. Our logistic regression analysis affirms that young age is the most important risk factor for pediculosis capitis. Moreover, female sex, large household size, living in wooden houses and the presence of animals were identified as being significantly associated with head lice infestation.
| Acknowledgments|| |
We thank the staff of the Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia (UPCH) in Lima, especially the biologist Marco Canales for his advice and support and the medical technologist Edhizon Trejo for the collaboration and his patience in the daily work in Barrios Altos.
We also thank the physicians of the Department of Dermatology of the UPCH, Dr. Lucie Puell, Dr. Francisco Bravo, Dr. Manuel Del Solar and Dr. Martνn Salomσn for their valuable advice throughout the study as well as Ms. B. Subirνa from the Casa Carrillo Maϊrtua for her help to get in contact with the population of Barrios Altos.
Furthermore, we want to thank all inhabitants of Barrios Altos for their participation, especially, Ana Ojeda and women who accompanied us during the field work.
We also thank Dr. Fernando Osores from the Colegio Mιdico del Perϊ and Dr. Αgueda Muρoz from the Universidad Catσlica de Santa Marνa (Arequipa, Perϊ) for providing access to some Peruvian publications about the topic.
This study was supported by a scholarship of the DAAD (German academic exchange service) for A.B. during his field work in Lima and by funds granted by Grόnenthal GmbH and Grόnenthal Peruana S.A. for the project "Scabiosis epidemiology and morbidity in low-income communities in Lima, Perϊ"; UPCH Register: SIDISI No. 50453.
| References|| |
|1.||Nutanson I, Steen CJ, Schwartz RA, Janniger CK. Pediculus humanus capitis: An update. Acta Dermatovenerol Alp Panonica Adriat 2008;17:147-54,156. |
|2.||Suleman M, Jabeen N. Head lice infestation in some urban localities of NWFP, Pakistan. Ann Trop Med Parasitol 1989;83:539-47. |
|3.||Heukelbach J, Wilcke T, Winter B, Feldmeier H. Epidemiology and morbidity of scabies and pediculosis capitis in resource-poor communities in Brazil. Br J Dermatol 2005;153:150-6. |
|4.||Catalá S, Junco L, Vaporaky R. Pediculus capitis infestation according to sex and social factors in Argentina. Rev Saude Publica 2005;39:438-43. |
|5.||Bachok N, Nordin RB, Awang CW, Ibrahim NA, Naing L. Prevalence and associated factors of head lice infestation among primary schoolchildren in Kelantan, Malaysia. Southeast Asian J Trop Med Public Health 2006;37:536-43. |
|6.||Falagas ME, Matthaiou DK, Rafailidis PI, Panos G, Pappas G. Worldwide prevalence of head lice. Emerg Infect Dis 2008;14:1493-4. |
|7.||Janniger CK, Kuflik AS. Pediculosis capitis. Cutis 1993;51:407-8. |
|8.||Mumcuoglu KY, Klaus S, Kafka D, Teiler M, Miller J. Clinical observations related to head lice infestation. J Am Acad Dermatol 1991;25:248-51. |
|9.||Heukelbach J, Feldmeier H. Ectoparasites - The underestimated realm. Lancet 2004;363:889-91. |
|10.||Speare R, Cahill C, Thomas G. Head lice on pillows, and strategies to make a small risk even less. Int J Dermatol 2003;42:626-9. |
|11.||Feldmeier H, Heukelbach J. Epidermal parasitic skin diseases: A neglected category of poverty-associated plagues. Bull World Health Organ 2009;87:152-9. |
|12.||Manrique P, Bricena V. Prevalence of pediculosis in the city of Camaná. Arequipa: Universidad Católica Santa María de Arequipa; 1990. |
|13.||Robles E. Socio-cultural factors and prevalence of pediculosis in students of primary education in the National College "César Vallejo". Lima, Peru: Universidad Peruana Cayetano Heredia; 1991. |
|14.||Yanqui W. Prevalence of pediculosis in the locality of Ciudad Nueva. Arequipa: Universidad San Agustín De Arequipa; 1992. |
|15.||Mostorino R, Anaya E, Menoya L, Rosas A. Identification of a new area of infection for rickettsia of the typhii group: Study of an outbreak of typhus in Huánuco. Rev Peruana Med Exp Salud Pública 2003;20:15-21. |
|16.||Abdel-Hafez K, Abdel-Aty MA, Hofny ER. Prevalence of skin diseases in rural areas of Assiut Governorate, Upper Egypt. Int J Dermatol 2003;42:887-92. |
|17.||Henderson CA. Skin disease in rural Tanzania. Int J Dermatol 1996;35:640-2. |
|18.||Feldmeier H. Pediculosis capitis. Kinder- und Jugendmedizin 2006;6:249-59. |
|19.||Cazorla D, Ruiz A, Acosta M. Clinical and epidemiological study of pediculosis capitis in schoolchildren from Coro, Venezuela. Invest Clin 2007;48:445-57. |
|20.||Toloza A, Vassena C, Gallardo A, González-Audino P, Picollo MI. Epidemiology of Pediculosis capitis in elementary schools of Buenos Aires, Argentina. Parasitol Res 2009;104:1295-8. |
|21.||Borges R, Mendes J. Epidemiological aspects of head lice in children attending day care centres, urban and rural schools in Uberlândia, central Brazil. Mem Inst Oswaldo Cruz 2002;97:189-92. |
|22.||Al-Maktari MT. Head louse infestations in Yemen: Prevalence and risk factors determination among primary schoolchildren, Al-Mahweet Governorate, Yemen. J Egypt Soc Parasitol 2008;38:741-8. |
|23.||Sim S, Lee IY, Lee KJ, Seo JH, Im KI, Shin MH, et al. A survey on head lice infestation in Korea (2001) and the therapeutic efficacy of oral trimethoprim/sulfamethoxazole adding to lindane shampoo. Korean J Parasitol 2003;41:57-61. |
|24.||Motovali-Emami M, Aflatoonian MR, Fekri A, Yazdi M. Epidemiological aspects of Pediculosis capitis and treatment evaluation in primary-school children in Iran. Pak J Biol Sci 2008;11:260-4. |
|25.||Nazari M, Fakoorziba MR, Shobeiri F. Pediculus capitis infestation according to sex and social factors in Hamedan, Iran. Southeast Asian J Trop Med Public Health 2006;37 Suppl 3:95-8. |
|26.||Oðuzkaya Artan M, Baykan Z, Koç AN. The prevalence of Pediculus capitis in students of eight primary schools in the rural area of the Kayseri province. Turkiye Parazitol Derg 2006;30:112-4. |
|27.||Bauer E, Jahnke C, Feldmeier H. Seasonal fluctuations of head lice infestation in Germany. Parasitol Res 2009;104:677-81. |
|28.||Ríos SM, Fernández JA, Rivas F, Sáenz ML, Moncada LI. Pediculosis prevalence and associated risk factors in a nursery school, Bogotá, Colombia. Biomedica 2008;28:245-51. |
|29.||Lonc E, Okulewicz A. Scabies and head-lice infestations in different environmental conditions of Lower Silesia, Poland. J Parasitol 2000;86:170-1. |
|30.||Burgess IF. Human lice and their management. Adv Parasitol 1995;36:271-342. |
|31.||Mumcuoglu KY, Meinking TA, Burkhart CN, Burkhart CG. Head louse infestations: The "no nit" policy and its consequences. Int J Dermatol 2006;45:891-6. |
|32.||Soultana V, Euthumia P, Antonios M, Angeliki RS. Prevalence of pediculosis capitis among schoolchildren in Greece and risk factors: A questionnaire survey. Pediatr Dermatol 2009;26:701-5. |
|33.||Kamiabi F, Nakhaei FH. Prevalence of pediculosis capitis and determination of risk factors in primary-school children in Kerman. East Mediterr Health J 2005;11:988-92. |
|34.||Nazari M, Saidijam M. Pediculus capitis infestation according to sex and social factors in Hamedan-Iran. Pak J Biol Sci 2007;10:3473-5. |
|35.||Balcioglu IC, Kurt O, Limoncu ME, Dinç G, Gümüº M, Kilimcioglu AA, et al . Rural life, lower socioeconomic status and parasitic infections. Parasitol Int 2007;56:129-33. |
|36.||Jahnke C, Bauer E, Hengge UR, Feldmeier H. Accuracy of diagnosis of pediculosis capitis: Visual inspection vs wet combing. Arch Dermatol 2009;145:309-13. |
|37.||Mumcuoglu KY, Friger M, Ioffe-Uspensky I, Ben-Ishai F, Miller J. Louse comb versus direct visual examination for the diagnosis of head louse infestations. Pediatr Dermatol 2001;18:9-12. |
Institute of Microbiology and Hygiene, Charité Universitätsmedizin Berlin, Campus Benjamin Franklin, Hindenburgdamm, 27, 12203 Berlin, Germany
Source of Support: None, Conflict of Interest: None
[Table 1], [Table 2], [Table 3]