| ORIGINAL ARTICLE |
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| Year : 2013 | Volume
: 5
| Issue : 2 | Page : 49-53 |
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Multiplex PCR for rapid diagnosis of gastrointestinal tuberculosis
Kusum Sharma1, Saroj Kant Sinha2, Aman Sharma3, Ritambra Nada4, Kaushal K Prasad2, Kapil Goyal1, Surinder Singh Rana2, Deepak Kumar Bhasin2, Meera Sharma1
1 Department of Medical Microbiology, Post Graduate Institute of Medical Education and Research, Chandigarh, India
2 Department of Gastroenterology, Post Graduate Institute of Medical Education and Research, Chandigarh, India
3 Department of Internal Medicine, Post Graduate Institute of Medical Education and Research, Chandigarh, India
4 Department of Pathology, Post Graduate Institute of Medical Education and Research, Chandigarh, India
Correspondence Address:
Kusum Sharma
Department of Medical Microbiology, Post Graduate Institute of Medical Education and Research, Chandigarh
India
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/0974-777X.112272
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Background: Rapid and specific diagnosis of gastrointestinal tuberculosis (GITB) is of utmost importance. Aim: To evaluate Multiplex PCR (MPCR) using MPB64 and IS6110 primers specific for M. tuberculosis for rapid diagnosis of GITB. Materials and Methods: MPCR was performed on colonoscopy biopsy specimens on 11 GITB confirmed (culture/AFB/histopathology was positive), 29 GITB suspected and 30 Non GITB (control group) patients. Results: MPB64 PCR had sensitivity and specificity of 90% and 100% for confirmed GITB cases. In 29 clinically diagnosed but unconfirmed GITB cases, MPCR was positive in 72.41%. MPCR was negative in all control group patients. The overall sensitivity and specificity of microscopy, culture, histopathology and MPCR was 5%, 2% 20% and 77.5% and 100%, 100%, 100% and 100% respectively. Conclusion: MPCR has good sensitivity and specificity in diagnosing gastrointestinal tuberculosis. |
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