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  Indian J Med Microbiol
 

Figure 1: Biomaterial development and characterization. (a) Flowchart representation of enzymatic processing of placenta and the observed biophysical features of the processed amnion scaffolds. (b) Masson's Trichrome staining of preprocessed amnion material showing amnion (red arrowhead) and chorion (asterisk) layers with collagen stained in deep blue, epithelial cell layer (black arrowhead) of amnion and lipid content of chorion stained in pink. (c) Scanning electron microscopy of preprocessed amnion showing a lipid-filled chorion that is clearly demarcated from collagen-filled amnion layer. (d) Histology staining of postprocessed amnion membrane, (i, ii) Masson's Trichrome staining of collagen-rich part of the amnion membrane with or without attached epithelial layer. Collagen is stained in deep blue, (iii) Verhoeff's elastic staining on amnion membrane showing cationic, anionic, and nonionic bonds with elastin in pink, (iv) Hematoxylin and eosin staining of postprocessed membrane showing nucleus of the epithelial cell layer of amnion shown in blue, rest of the tissue is in pink color, (v) Periodic acid-Schiff staining showing polysaccharide content of the postprocessed amnion membrane. (e) Scanning electron microscopy on posttreated amnion showing a multigroove structure (i) that is regularly spaced (ii) having hollow interior (iii, iv). (f) Postprocessed amnion processed as a thin sheet. (g) Cell viability measurement of fibroblast grown without (Control) or without amnion scaffold

Figure 1: Biomaterial development and characterization. (a) Flowchart representation of enzymatic processing of placenta and the observed biophysical features of the processed amnion scaffolds. (b) Masson's Trichrome staining of preprocessed amnion material showing amnion (red arrowhead) and chorion (asterisk) layers with collagen stained in deep blue, epithelial cell layer (black arrowhead) of amnion and lipid content of chorion stained in pink. (c) Scanning electron microscopy of preprocessed amnion showing a lipid-filled chorion that is clearly demarcated from collagen-filled amnion layer. (d) Histology staining of postprocessed amnion membrane, (i, ii) Masson's Trichrome staining of collagen-rich part of the amnion membrane with or without attached epithelial layer. Collagen is stained in deep blue, (iii) Verhoeff's elastic staining on amnion membrane showing cationic, anionic, and nonionic bonds with elastin in pink, (iv) Hematoxylin and eosin staining of postprocessed membrane showing nucleus of the epithelial cell layer of amnion shown in blue, rest of the tissue is in pink color, (v) Periodic acid-Schiff staining showing polysaccharide content of the postprocessed amnion membrane. (e) Scanning electron microscopy on posttreated amnion showing a multigroove structure (i) that is regularly spaced (ii) having hollow interior (iii, iv). (f) Postprocessed amnion processed as a thin sheet. (g) Cell viability measurement of fibroblast grown without (Control) or without amnion scaffold