Journal of Global Infectious Diseases

ORIGINAL ARTICLE
Year
: 2018  |  Volume : 10  |  Issue : 2  |  Page : 58--66

Proteomic analysis of avian influenza A (H7N9) patients within a family cluster


Yang Zheng1, Xiaomin Lou2, Peng Yang1, Weixian Shi1, Yanda Chu3, Meishuang Yan3, Cuicui Jiang3, Di Wu3, Yang Pan1, Jiachen Zhao1, Yang Li3, Yusheng Dong3, Lijuan Chen1, Siqi Liu4, Quanyi Wang1 
1 Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control; Research Center for Preventive Medicine of Beijing, Beijing, China
2 Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China
3 Beijing Protein Innovation, Beijing, China
4 Beijing Institute of Genomics, Chinese Academy of Sciences; Beijing Protein Innovation, Beijing, China

Correspondence Address:
Dr. Quanyi Wang
Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, No. 16 He Pingli Middle Street, Dongcheng District, Beijing 100013
China

Background: To date, there is limited information on the progression of human infections of avian influenza virus A (H7N9). This study investigated differential blood protein profiling of a H7N9-infected family cluster to find a slice of crucial proteins concerning disease attack and virus clearance.Materials and Methods: Plasma samples from one family cluster (including one index case and one asymptomatic case) were collected at four time points. The protein profiles were identified by isobaric tagging for relative and absolute quantification-based quantitative differential LC/MS/MS, and their functional annotations were analyzed by PANTHER and STRING tools. Results: A total of 1257 nonredundant proteins were identified from 3027 unique peptides. Three differential protein profiles for each subject were generated by comparing relative protein abundance between samples of each of the first three time points and the last time point. Gene ontology analysis indicated that differential protein profiles for the two cases were mainly enriched in the biological processes of response to stimulus, immunity, blood coagulation, lipid transport, and cell adhesion. Two groups of proteins with an upward or downward expression change according to the postinfection time points were detected for each case. STRING analysis further indicated that the hubs in the network of these time-dependent proteins were mostly apolipoproteins. Conclusions: Significant perturbation of the response upon viral infection occurred immediately after confirmation of H7N9 virus infection. The differential protein profiles shed further light on distinguishing the index case from the asymptomatic one. Furthermore, apolipoproteins may play an important role in the progression of the disease.


How to cite this article:
Zheng Y, Lou X, Yang P, Shi W, Chu Y, Yan M, Jiang C, Wu D, Pan Y, Zhao J, Li Y, Dong Y, Chen L, Liu S, Wang Q. Proteomic analysis of avian influenza A (H7N9) patients within a family cluster.J Global Infect Dis 2018;10:58-66


How to cite this URL:
Zheng Y, Lou X, Yang P, Shi W, Chu Y, Yan M, Jiang C, Wu D, Pan Y, Zhao J, Li Y, Dong Y, Chen L, Liu S, Wang Q. Proteomic analysis of avian influenza A (H7N9) patients within a family cluster. J Global Infect Dis [serial online] 2018 [cited 2019 Aug 19 ];10:58-66
Available from: http://www.jgid.org/article.asp?issn=0974-777X;year=2018;volume=10;issue=2;spage=58;epage=66;aulast=Zheng;type=0