Journal of Global Infectious Diseases

LETTER TO EDITOR
Year
: 2013  |  Volume : 5  |  Issue : 2  |  Page : 86--87

Diagnosis of extrapulmonary tuberculosis by polymerase chain reaction for mpb64 gene: An evaluation in a hospital based study


Sushma Pednekar1, Arvind V Bhore2, Vrishali A Muley2, Dnyaneshwari P Ghadage2,  
1 Department of Microbiology, B. J. Medical College and Sassoon, General Hospitals, Pune, India
2 Smt. Kashibai Navale Medical College and General Hospitals, Mumbai-Pune, Bypass, Pune, India

Correspondence Address:
Vrishali A Muley
Smt. Kashibai Navale Medical College and General Hospitals, Mumbai-Pune, Bypass, Pune
India




How to cite this article:
Pednekar S, Bhore AV, Muley VA, Ghadage DP. Diagnosis of extrapulmonary tuberculosis by polymerase chain reaction for mpb64 gene: An evaluation in a hospital based study.J Global Infect Dis 2013;5:86-87


How to cite this URL:
Pednekar S, Bhore AV, Muley VA, Ghadage DP. Diagnosis of extrapulmonary tuberculosis by polymerase chain reaction for mpb64 gene: An evaluation in a hospital based study. J Global Infect Dis [serial online] 2013 [cited 2020 Sep 20 ];5:86-87
Available from: http://www.jgid.org/text.asp?2013/5/2/86/112283


Full Text

Sir,

Extrapulmonary tuberculosis (EPTB) often possesses a diagnostic dilemma. Microscopy and culture have proved to be insensitive techniques for diagnosis of EPTB. [1] The present study was conducted to evaluate the role of polymerase chain reaction (PCR) in the early diagnosis of clinically suspected cases of EPTB.

A total of 50 clinical specimens comprising pleural fluid, cerebrospinal fluid, ascitic fluid, fine needle aspiration biopsy, pus and biopsy from clinically suspected EPTB cases were processed and followed by conventional methods [2],[3] and PCR using MPB64 primer.

Tuberculous pleural effusion (68%) was found to be the commonest clinical presentation of EPTB. Overall, PCR could detect EPTB in 62% cases. Microscopy and culture could detect 16% and 20% EPTB cases respectively. PCR was positive in all the tissue samples suggestive of tuberculosis on histopathological examination. Of the 39 EPTB patients who responded to antituberculosis treatment, 31 patients were PCR positive.

PCR using MPB64 had a significant advantage over the conventional methods in diagnosing EPTB cases.

References

1Kesarwani RC, Pandey A, Misra A, Singh AK. Polymerase chain reaction: Its comparison with conventional techniques for diagnosis of extra-pulmonary tubercular diseases. Indian J Surg 2004;66:84-8.
2Koneman EW, Allen SD, Janda WM. Mycobacteria. In colour atlas and textbook of Diagnostic Microbiology. 5th ed. Lippincott: Philadelphia; 1979. p. 404-27.
3Grange JM. The Mycobacteria. In: Topley and Wilson's Principles of bacteriology, virology and immunity. Systemic bacteriology. In: Wilson G, Dick HM, Miles A, Parker NT, editors. 8 th ed. Vol 2. London: Hodder and Stoughton; 1990. p. 74-101.