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LETTER TO EDITOR  
Year : 2019  |  Volume : 11  |  Issue : 3  |  Page : 129-130
Follow-up of a case of cryptosporidiosis in a toddler from Mexico: Response to the treatment


Department of Biological Chemistry Sciences and Department of Medicine and Health Sciences, University of Sonora, Hermosillo, Sonora, Mexico

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Date of Web Publication26-Aug-2019
 

How to cite this article:
Urrea-Quezada A, Sotelo-Cruz N, González-Díaz M, Valenzuela O. Follow-up of a case of cryptosporidiosis in a toddler from Mexico: Response to the treatment. J Global Infect Dis 2019;11:129-30

How to cite this URL:
Urrea-Quezada A, Sotelo-Cruz N, González-Díaz M, Valenzuela O. Follow-up of a case of cryptosporidiosis in a toddler from Mexico: Response to the treatment. J Global Infect Dis [serial online] 2019 [cited 2019 Sep 22];11:129-30. Available from: http://www.jgid.org/text.asp?2019/11/3/129/265392




Sir,

There are approximately 8 million annual deaths of children under 5 years of age worldwide, and diarrhea is associated with 10.5% of these deaths.[1]Cryptosporidium species are major pathogens that cause moderate-to-severe diarrhea in children in resource-poor settings. This study reports the case of a male patient of 17 months of age with a diarrheic episode for 10 days with liquid evacuations (without mucus or blood) 7–10 times per 24 h. The toddler was treated with trimethoprim sulfamethoxazole and probiotics. Despite the treatment, the patient continued to have diarrhea, and three consecutive stool samples were collected to look for the most prevalent intestinal parasites in the region.[2],[3],[4],[5]

The stool samples were positive for Cryptosporidium parvum (IIaA15G2R1) [Figure 1] and negative for the other parasites. The identification of genotype and subtype was confirmed by sequence analysis of the polymerase chain reaction (PCR) product. The toddler was treated with secnidazole 20 mg/kg and nitazoxanide 7.5 mg/kg every 12 h for 3 days. After 2 weeks, the stool samples were of normal consistency, but PCR was positive to C. parvum and the treatment was repeated. Twenty days later, the samples were negative. Follow-up was finished 2 months later, when the last three samples were PCR negative. This is a follow-up report of a case of cryptosporidiosis by molecular diagnosis from the moment of the identification of the parasite C. parvum until the stool samples were negative. Therefore, the diagnosis and monitoring of cases until the elimination of the parasite is essential to determine the effectiveness in the management of the infection or reinfections.
Figure 1: Polymerase chain reaction products of the gp60 gene of Cryptosporidium spp. Lane 1, molecular markers (bp); lane 2, Cryptosporidium parvum control; lanes 3–5, samples analyzed before treatment; lane 6, samples analyzed after 15 days of treatment

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Declaration of patient consent

The authors certify that they have obtained all appropriate patient consent form. Informed consent was obtained from the mother of the patient after the objectives of the study were clearly explained. The clinical characteristics of the patients were obtained with prior authorization from the patient's clinical files by the treating physician.

Acknowledgment

Alejandro and Mariana received a scholarship from CONACYT and are enrolled in the PhD Program of the Department of Biological Chemistry Sciences, University of Sonora.

Financial support and sponsorship

This work was supported by Consejo Nacional de Ciencia y Tecnología, Fondo Sectorial de Investigación para la Educación (grant number 258454).

Conflict of interest

There are no conflicts of interest.



 
   References Top

1.
Ryan U, Fayer R, Xiao L. Cryptosporidium species in humans and animals: Current understanding and research needs. Parasitology 2014;141:1667-85.  Back to cited text no. 1
    
2.
Villegas-Gómez I, Martínez-Hernández F, Urrea-Quezada A, González-Díaz M, Durazo M, Hernández J, et al. Comparison of the genetic variability of blastocystis subtypes between human carriers from two contrasting climatic regions of México. Infect Genet Evol 2016;44:334-40.  Back to cited text no. 2
    
3.
Valenzuela O, Morán P, Ramos F, Cardoza JI, García G, Valadez A, et al. Two different chitinase genotypes in a patient with an amebic liver abscess: A case report. Am J Trop Med Hyg 2009;80:51-4.  Back to cited text no. 3
    
4.
Eligio-García L, Cortes-Campos A, Cota-Guajardo S, Gaxiola S, Jiménez-Cardoso E. Frequency of Giardia intestinalis assemblages isolated from dogs and humans in a community from Culiacan, Sinaloa, Mexico using beta-giardin restriction gene. Vet Parasitol 2008;156:205-9.  Back to cited text no. 4
    
5.
Valenzuela O, González-Díaz M, Garibay-Escobar A, Burgara-Estrella A, Cano M, Durazo M, et al. Molecular characterization of Cryptosporidium spp. in children from Mexico. PLoS One 2014;9:e96128.  Back to cited text no. 5
    

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Correspondence Address:
Dr. Olivia Valenzuela
Department of Biological Chemistry Sciences, University of Sonora, Blvd. Luis Encinas and Rosales, Hermosillo 83000, Sonora
Mexico
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/jgid.jgid_119_18

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2008 Journal of Global Infectious Diseases | Published by Wolters Kluwer - Medknow
Online since 10th December, 2008