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   Table of Contents     
LETTER TO EDITOR  
Year : 2019  |  Volume : 11  |  Issue : 3  |  Page : 127-129
Universal primers as a potential tool for the detection of emerging flaviviruses


1 Department of Research, Cancer State Institute, Ministry of Health, Colima, Mexico
2 Department of Internal Medicine, University Regional Hospital, Ministry of Health, Colima, Mexico
3 Department of Audiology, Mexican Social Security Institute, University of Colima, Colima, Mexico
4 Department of Public Health, School of Medicine, University of Colima, Colima, Mexico
5 Department of Veterinary Public Health, Regional Animal Health Laboratory, Colima, Mexico
6 Department of Health Sciences, Center for Biodefense and Global Infectious Diseases, Colima, Mexico

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Date of Web Publication26-Aug-2019
 

How to cite this article:
Delgado-Enciso I, Espinoza-Gómez F, Verján-Carrillo EJ, Ceja-Espiritu G, Rios-Flores PA, Lizama-Munguía T, Salazar-Barragán JA, Soto-Castellano JB, Valle-Reyes S, López-Lemus UA. Universal primers as a potential tool for the detection of emerging flaviviruses. J Global Infect Dis 2019;11:127-9

How to cite this URL:
Delgado-Enciso I, Espinoza-Gómez F, Verján-Carrillo EJ, Ceja-Espiritu G, Rios-Flores PA, Lizama-Munguía T, Salazar-Barragán JA, Soto-Castellano JB, Valle-Reyes S, López-Lemus UA. Universal primers as a potential tool for the detection of emerging flaviviruses. J Global Infect Dis [serial online] 2019 [cited 2019 Sep 15];11:127-9. Available from: http://www.jgid.org/text.asp?2019/11/3/127/265395




Sir,

Flaviviruses (FVs) are arthropod-borne viruses that affect humans in tropical regions worldwide.[1] Previous reports[1],[2] have shown the importance of implementing FV novel detection strategies in tropical regions, such as the use of universal primers that enable a simultaneous identification of these pathogens in a single polymerase chain reaction (PCR).[1],[2],[3]

We analyzed 462 blood samples from febrile patients in western Mexico. FV identification was carried out through a PCR assay using universal primers validatedin vitro by Maher-Sturgess et al. A total of 74 blood samples amplified with different amplicon sizes similar to those previously described.[4] Our DNA sequencing analysis showed the identification of dengue virus serotype 1 in 31 blood samples,[5] and the remaining 43 amplicons corresponded to different human gene regions [Table 1], representing 9.3% of all the samples analyzed.
Table 1: Polymerase chain reaction results using the universal primers, Flav100F and Flav200R, corresponding to different sizes of amplicons of human genes

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Undoubtedly, the use of nonvalidated universal PCR primers in natural hosts represents a risk for obtaining unreliable results in animal and human populations due to the diversity of genomes. The development of universal PCR primers for FV detection is a potential tool for its diagnosis, especially in tropical regions where FVs frequently coexist, so febrile symptoms associated with FVs easily could result in a diagnostic confusion. This clinical confusion could interfere on the deployment of effective solutions for the detection, prevention, and control of emergent FV outbreaks in timely manner; therefore, the control of FV diseases could be hampered if effective and reliable detection strategies are not implemented.

Acknowledgments

The authors would like to thank the National Council for Science and Technology (Federal Government, Mexico) for the financial assistance provided through CONACYT-FORDECyT grant #2009/1-117535.

Financial support and sponsorship

This work was supported by the National Council for Science and Technology of Mexico (CONACYT-FORDECyT grant #2009/1-117535).

Conflicts of interest

There are no conflicts of interest.



 
   References Top

1.
Brooks T, Roy-Burman A, Tuholske C, Busch MP, Bakkour S, Stone M, et al. Real-time evolution of zika virus disease outbreak, Roatán, Honduras. Emerg Infect Dis 2017;23:1360-3.  Back to cited text no. 1
    
2.
Espinoza-Gómez F, López-Lemus AU, Rodriguez-Sanchez IP, Martinez-Fierro ML, Newton-Sánchez OA, Chávez-Flores E, et al. Detection of sequences from a potentially novel strain of cell fusing agent virus in Mexican stegomyia (Aedes) aegypti mosquitoes. Arch Virol 2011;156:1263-7.  Back to cited text no. 2
    
3.
Scaramozzino N, Crance JM, Jouan A, DeBriel DA, Stoll F, Garin D, et al. Comparison of flavivirus universal primer pairs and development of a rapid, highly sensitive heminested reverse transcription-PCR assay for detection of flaviviruses targeted to a conserved region of the NS5 gene sequences. J Clin Microbiol 2001;39:1922-7.  Back to cited text no. 3
    
4.
Maher-Sturgess SL, Forrester NL, Wayper PJ, Gould EA, Hall RA, Barnard RT, et al. Universal primers that amplify RNA from all three flavivirus subgroups. Virol J 2008;5:16.  Back to cited text no. 4
    
5.
Delgado-Enciso I, López-Lemus UA, Valcarcel-Gamiño JA, Rodriguez-Sanchez IP, Valle-Reyes S, Martinez-Fierro ML, et al. Dengue virus-1 NS5 genetic variant associated with a severe clinical infection: Possible reduction of the innate immune response by inhibition of interferon type 1 and the janus kinase-signal transducer and activator of transcription signaling pathway. Int J Mol Med 2018;41:2263-9.  Back to cited text no. 5
    

Top
Correspondence Address:
Dr. Uriel A López-Lemus
Rodolfo Chávez Carrillo, S/N, Santa Teresa, 28078 Colima
Mexico
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/jgid.jgid_137_18

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2008 Journal of Global Infectious Diseases | Published by Wolters Kluwer - Medknow
Online since 10th December, 2008